The examiner hands each card to the subject and asks the test subject what he or she sees in the inkblot. The test administrator, or examiner, has a stack of 47 cards with inkblots (45 test cards and 2 practice cards) face down in front of him or her. Unlike the Rorschach, the Holtzman is a standardized measurement with clearly defined objective scoring criteria. The test was designed to overcome some of the deficiencies of its famous predecessor, the Rorschach Inkblot Test. The HIT, developed by psychologist Wayne Holtzman and colleagues, was introduced in 1961. If the examiner is not well-trained in psychometric evaluation, subjective interpretations may affect the outcome of the test. Some consider projective tests to be less reliable than objective personality tests. The HIT should be administered and interpreted by a trained psychologist, psychiatrist, or appropriately trained mental health professional. Psychometric testing requires a clinically trained examiner. It is sometimes used as a diagnostic tool in assessing schizophrenia, depression, addiction, and character disorders.

The HIT is used to assess the personality structure of a test subject. medical-conditions/sexual-and-reproductive-health/hiv-aids/diagnosis/western-blot-test.The Holtzman Inkblot Technique (HIT) is a projective personality assessment test for persons ages five and up. Updated CDC recommendation for serologic diagnosis of Lyme disease.cdc.gov/hiv/basics/hiv-testing/test-types.html Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Western blot: Technique, theory, and troubleshooting. aboutAPHL/publications/Documents/ID_HIV-1-1-WesternBlotBrief_62015.pdf Limitations for the use of HIV-1 western blot in plasma/serum.5/case-of-month-possible-false-positive-hiv-test/ Case of the month: Possible false positive HIV test.You can learn more about how we ensure our content is accurate and current by reading our editorial policy. Healthline has strict sourcing guidelines and relies on peer-reviewed studies, academic research institutions, and medical associations. Since it can take several weeks or months before antibodies are found in blood, the Western blot test may not always be reliable. If it causes a change in color, antibodies to a specific infection have been detected. The proteins are placed onto blotting paper that’s made from a material such as nitrocellulose.Īn enzyme is added to the paper. The Western blot uses a procedure called gel electrophoresis to identify and separate proteins by molecular weight and length. Antigens stimulate your immune system to push out antibodies in an effort to fight the disease.

If you develop a viral, fungal, or bacterial infection, your body will produce proteins called antigens in response. The Western blot test looks for the antibodies against an infection, not the infection itself. If you get a positive result for Lyme disease or HIV after taking an enzyme-linked immunosorbent assay (ELISA) test, your doctor may recommend a Western blot test to you. It was invented in 1979 to identify ribosomal RNA binding proteins. The Western blot test is also known as a protein immunoblot test or immunoblotting.

Ink blot test